General Functions

Functions for general processing workflow.

tsr_explorer()

TSRexploreR Class

format_counts()

Format Counts

annotate_features()

Annotate Features

plot_threshold_exploration()

Plot Threshold Exploration

apply_threshold()

Apply a threshold to TSSs or TSRs

merge_samples()

Merge Samples

normalize_counts()

Normalize TSS counts

tss_clustering()

TSS Clustering

TSS/TSR Metrics

Functions to explore TSS and/or TSR metrics.

associate_with_tsr()

Associate TSSs

mark_dominant()

Mark Dominant

tsr_metrics()

TSR Metrics

plot_tsr_metric()

Plot TSR Metrics

BAM Processing

Functions to import and process BAM files.

G_correction()

Correct G artifact

import_bams()

Import BAMs

softclip_composition()

Composition of Soft-clipped Bases

softclip_histogram()

Number of soft-clipped bases.

tss_aggregate()

Aggregate TSSs

TSS/TSR Import and Export

Functions to import and export TSSs and TSRs.

tss_import()

Import TSSs

tsr_import()

Import TSRs

tss_export()

Export TSSs

tsr_export()

Export TSRs

Accessor Functions

Functions to access data within TSRexploreR object.

get_annotation()

Get Genome Annotation

get_assembly()

Get Genome Assembly

get_counts()

Get TSS or TSR count tables.

get_diff_model()

Get Differential TSS/TSR models

get_diff_results()

Get Differential TSS/TSR results

get_granges()

Get TSS or TSR GRanges.

get_sample_sheet()

Get Sample Sheet

get_shifting_results()

Get Shifting Results

TSR Shifting

Functions specific to TSR shifting analysis.

tss_shift()

TSS Shifting

plot_shift_rank()

Shifting Rank Plot

plot_shift_count()

Shift Count Plot

TSS/TSR Plotting Functions

Plotting functions for TSS and TSR data.

gene_tracks()

Gene Tracks

plot_correlation()

Plot Sample Correlation

plot_density()

Density Plots

plot_detected_features()

Genes/Transcripts Detected

plot_genomic_distribution()

Genomic Distribution

plot_heatmap()

Plot Heatmap

plot_reduction()

Dimensionality Reduction.

TSS Specific Plots

Plotting functions specific to TSS data.

plot_dinucleotide_frequencies()

Dinucleotide Analysis

plot_sequence_colormap()

Plot Sequence Color Map

plot_sequence_logo()

Generate Sequence Logo

Differential TSSs/TSRs

Functions for differential TSS/TSR analysis and plotting.

fit_de_model()

Differential feature analysis

differential_expression()

Analyze Differential Features

export_for_enrichment()

Export to clusterProfiler

plot_ma()

MA Plot

plot_num_de()

Plot DE Numbers

plot_volcano()

Volcano Plot

Data Conditionals

Accessory functions to condition data for plotting

conditionals()

Data Conditions Input

ordering()

Ordering

quantiling()

Quantiling

Datasets

Internal datasets.

TSSs

Yeast STRIPE-seq TSSs

TSSs_reduced

STRIPE-seq TSSs YDR077W (Diamide)

Internal Functions

Internal functions that should be ignored by end user

ShiftScores()

Shift Score

common_params()

Function to hold common parameters.

.count_features()

Calculate Feature Counts

.count_matrix()

Generate Count Matrix.

.de_status()

Mark DE Status

.out_of_bounds_index()

Get index of out of bounds ranges.

.prepare_annotation()

Prepare Genome Annotation

.prepare_assembly()

Prepare Genome Assembly

condition_data()

Data Conditionals

extract_counts()

Extract Counts

extract_de()

Extract Differential Gene Sets

extract_matrix()

Extract Count Matrices

preliminary_filter()

Preliminary Filter

retrieve_seqs()

Retrieve Sequences

set_count_slot()

Set Slot Data

tsr_experiment()

Add TSRs

tss_experiment()

Add TSSs